| Serie 1 |
| Arianna Abigail Botton Soto | Academia de Barranco | 5 | 1:01.21 | 1 q |
| Luciana Gabriela Fernandez Pazmiño | Deportivo Running-K Cusco | 3 | 1:03.55 | 4 q |
| Kimberly Antonella Borja Cordova | Clínicas Deportivas | 6 | 1:05.87 | 7 q |
| Yashira Lesly Cajas Cori | Atletic Deportmanu | 7 | 1:12.51 | 8 |
| Paula Leonor Cox | Deportivo Centinelas SAC | 4 | 1:04.68 | (F.C.) q |
| Serie 2 |
| Rebeca Viviana Vasquez Maldonado | Academia de Barranco | 5 | 1:01.79 | 2 q |
| Andrea Fatima Gomez Lazo | Olimpia AQP | 4 | 1:03.47 | 3 q |
| Vania Lizzeth Ramirez Farfan | Clínicas Deportivas | 3 | 1:04.78 | 5 q |
| Flavia Alejandra Vigil Lucero | Atletismo Scorpio | 6 | 1:05.12 | 6 q |
