| Serie 1 |
| Vania Lizzeth Ramirez Farfan | Makabe Club | 4 | 1:03.74 | 6 q |
| María Fernanda Pastor Núñez | Yumina | 6 | 1:09.38 | 12 |
| Ana Paola Diaz Leiva | Apolo Athletic Club | 8 | 1:11.35 | 14 |
| Samantha Alexia Eimi Matos Tsukazan | AELU | 3 | 1:14.21 | 15 |
| Yudy Iris Quispe Chambilla | Atlético JASVIC | 7 | 1:14.99 | 16 |
| Flavia Alejandra Vigil Lucero | Atletismo Scorpio | 5 | DQ | |
| Serie 2 |
| Luciana Gabriela Fernandez Pazmiño | Deportivo Running-K Cusco | 4 | 1:01.55 | 2 q |
| Ximena Vasquez Rodriguez | Marcon Athletic Club | 8 | 1:01.65 | 3 q |
| Kimberly Antonella Borja Cordova | Makabe Club | 5 | 1:02.50 | 4 q |
| Anayelly Belen Mio Aldana | Atletismo Sican | 7 | 1:06.58 | 8 q |
| Shantall Sammy Gonzales Gutierrez | Féniz | 3 | 1:08.92 | 11 |
| Jhenifer Maley Guerra Escobedo | Los Rosales | 6 | DNS | |
| Serie 3 |
| Daniela Alejandra Salazar Burga | Atletismo Chiclayo | 6 | 1:01.49 | 1 q |
| Paula Leonor Cox | Deportivo Centinelas SAC | 4 | 1:03.66 | 5 q |
| Luciana Sareli Traverso Rojas | Atletismo Chiclayo | 3 | 1:05.89 | 7 q |
| Ivana-Maria Antonova Koleva | Academia de Barranco | 7 | 1:08.18 | 9 |
| Heidy Stefany Céspedes Apaestegui | Deportivo Los Gavilanes | 5 | 1:08.72 | 10 |
| Tiffany Breisseth Gutierrez Cárdenas | Asociación Deportiva Cultural Amazonas | 2 | 1:10.39 | 13 |
