| Serie 1 V.V. +1.0 |
| Luciana Gabriela Fernandez Pazmiño | Deportivo Running-K Cusco | 4 | 27.18 | 1 q |
| Tracy Alondra Joshelin Paucara Flores | Los Rosales | 3 | 28.92 | 11 |
| Luciana Cateriano Medina | Féniz | 2 | 28.96 | 12 |
| Mikaela Ramos Diaz | Makabe Club | 8 | 29.36 | 14 |
| Ivana-Maria Antonova Koleva | Academia de Barranco | 5 | 29.54 | 16 |
| Jimena Zapater Farfan | IED San Jerónimo | 6 | 31.66 | 22 |
| Flavia Yari Alvaro Mori | Atlético Punta Hermosa | 1 | 33.00 | 27 |
| Dennis Alessandra Mendoza Tagle | Alto Rendimiento Deportivo Moreno y Guzmán SAC | 7 | DNS | |
| Serie 2 V.V. +1.1 |
| Daniela Alejandra Salazar Burga | Atletismo Chiclayo | 8 | 27.30 | 2 q |
| Kimberly Antonella Borja Cordova | Makabe Club | 7 | 27.73 | 4 q |
| Vania Lizzeth Ramirez Farfan | Makabe Club | 4 | 27.75 | 5 q |
| Valentina Silva Aparcana | Makabe Club | 6 | 28.21 | 7 q |
| Maylin De Los Angeles Lopez Cordova | Escuela Formación Deportiva | 1 | 29.24 | 13 |
| Heidy Stefany Céspedes Apaestegui | Deportivo Los Gavilanes | 3 | 29.51 | 15 |
| Jhenifer Maley Guerra Escobedo | Los Rosales | 2 | DNS | |
| Nicole Andrea Garcia Montoya | Atletismo Scorpio | 5 | DNS | |
| Serie 3 V.V. +0.9 |
| Adriana Lucia Chavez Valdiviezo | Runners Tumbes | 5 | 27.70 | 3 q |
| Luciana Sareli Traverso Rojas | Atletismo Chiclayo | 2 | 28.15 | 6 q |
| Analucia Tarrillo Saavedra | Atletismo Sican | 7 | 28.86 | 10 |
| Irix Giovanna Marin Paredes | Los Rosales | 4 | 29.82 | 17 |
| Gabriela Zharick Paucar Cornejo | Apolo Athletic Club | 8 | 30.32 | 19 |
| Brithany Grisell Cacsire De La Cruz | Academia de Barranco | 6 | 31.84 | 23 |
| Anyela Mercedes Chacon Arana | Féniz | 3 | 31.97 | 24 |
| Serie 4 V.V. +0.9 |
| Flavia Alejandra Vigil Lucero | Atletismo Scorpio | 4 | 28.25 | 8 q |
| Mariana Carolina Minchola Angulo | Team Lau | 8 | 28.63 | 9 |
| Camila Córdova Pilco | Atletismo La Molina | 7 | 30.04 | 18 |
| Maria Silvana Yaya Dominguez | Avia Sport Sac | 6 | 31.12 | 20 |
| Valeria Jimena Panta Namo | Del Norte | 3 | 31.44 | 21 |
| Francesca Merello Gonzales | CEDE | 2 | 32.15 | 25 |
| Yadhira Naylee Mercedes Validez Romero | Escuela Formación Deportiva | 5 | 32.36 | 26 |
