| Serie 1 |
| Jacoba Liliana Goicochea Becerra | Deportivo Los Gavilanes | 2:26.00 | 1 q |
| Ximena Vasquez Rodriguez | Marcon Athletic Club | 2:27.32 | 3 q |
| Anita Yuceli Poma Mendoza | Asociación Deportiva Atlético Felipides | 2:31.26 | 5 q |
| Emilia Fabiana Martinez Pinto | Marcon Athletic Club | 2:32.88 | 6 q |
| Scoly Tatiana Lopez Cordova | Escuela Formación Deportiva | 2:33.99 | 7 q |
| Shantall Sammy Gonzales Gutierrez | Féniz | 2:35.38 | 8 q |
| Soury Marina Flores Guillen | Avia Sport Sac | 2:36.52 | 11 |
| Ana Paola Diaz Leiva | Apolo Athletic Club | 2:48.45 | 15 |
| Adriana Isabel Maguiña Ticona | Escuela Formación Deportiva | 2:57.69 | 17 |
| Serie 2 |
| Luciana Beatriz Hilario Reyes | Atletismo Rivera Huancayo | 2:27.26 | 2 q |
| Melany Florentina Cayo Mamani | Atlético JASVIC | 2:30.88 | 4 q |
| Lisseth Janice Varillas Davalos | Atlético Ilbamir | 2:35.55 | 9 |
| Nohelia Silvina Luque Chambi | Deportivo Athletic Puno Runners | 2:35.90 | 10 |
| Cielo Marisel Del Milagro Rojas Pérez | Atletismo Chiclayo | 2:41.05 | 12 |
| Anayelly Belen Mio Aldana | Atletismo Sican | 2:44.47 | 13 |
| Gladys Beatriz Mendoza Mamani | Atlético JASVIC | 2:48.25 | 14 |
| Nadja Valentina Landeo Castro | Alborada Track and Field | 2:51.41 | 16 |
| Yudy Iris Quispe Chambilla | Atlético JASVIC | 3:05.19 | 18 |
