| Serie 1 |
| Dianellys Del Carmen Indriago Lista | Academia de Barranco | 4 | 1:02.74 | 1 q |
| Luciana Rivera Pezo | AELU | 3 | 1:04.14 | 3 q |
| Ariana María Del Castillo Pérez | Monteprincipe | 2 | 1:05.24 | 4 q |
| Luciana Mariel Mogrovejo Galindo | Atlético Ilbamir | 5 | 1:10.44 | 16 |
| Mayta Silva Jasmin Jade | Atlético Ilbamir | 6 | 1:22.94 | 27 |
| Micaela Carmen Hancco Mendoza | Multisports | 7 | DNS | |
| Mora Cordero Mitrani | Atletismo Valentina | 8 | DNS | |
| Serie 2 |
| Kiara Sofia Castro Eyzaguirre | Deportivo Dynamo Sport | 7 | 1:03.68 | 2 q |
| Rafaela Higa Suejiro | Atletismo Chiclayo | 8 | 1:07.17 | 10 |
| Briana Huaranga Mattos | Marcon Athletic Club | 6 | 1:07.64 | 12 |
| Korina Collas Canario | Team Chile | 4 | 1:08.26 | 14 |
| Sofia Fernanda Barrueta Lanfranco | Reina de los Ángeles | 3 | 1:11.66 | 18 |
| Ivana Padme Llerena Limazca | IED FAP Raúl Leguia Drago | 5 | 1:12.55 | 20 |
| Nicole Alexandra Olivera Picón | Atletismo Chiclayo | 2 | 1:14.42 | 22 |
| Serie 3 |
| Karina Valdeiglesias Tinta | Deportivo Chasquis de Sicuani | 4 | 1:06.45 | 8 q |
| Damaris Lucia Velasquez De La Mata | G Coach SAC | 8 | 1:07.59 | 11 |
| Briana Ossio Mosquera | Marcon Athletic Club | 3 | 1:10.35 | 15 |
| Natalia Jimenez Ruiz | Regatas | 5 | 1:11.34 | 17 |
| Maria Fernanda Iruyari Arbildo | Atletas Pisqueños | 6 | 1:22.75 | 26 |
| Mariana Beatriz Tabata Vasquez | Avia Sport Sac | 2 | DNS | |
| Jimena Paredes Guzman | Coar Deportivo Ica | 7 | DNS | |
| Serie 4 |
| Luciana Mathews Ocampo | Rojas Sport | 5 | 1:05.89 | 7 q |
| Brenda Kamila Fernandez Guevara | Alborada Track and Field | 4 | 1:06.62 | 9 |
| Akemi Monterroso Sota | AELU | 3 | 1:08.02 | 13 |
| Araceli Isabella Velarde Munayco | Atlético Talentos Santos | 2 | 1:12.77 | 21 |
| Fabiana Jesus Castillo Fajardo | Runners Tumbes | 7 | 1:16.59 | 23 |
| Keyla Steffany Simarra Vasquez | Deportivo Estudiantil República de Venezuela | 8 | 1:19.18 | 25 |
| Paula Nicole Cespedes Apaestegui | Deportivo Los Gavilanes | 6 | DNS | |
| Serie 5 |
| Rafaela Luana Gutierrez Orellana | Féniz | 4 | 1:05.35 | 5 q |
| Luisa Gabriela Chacon Arana | Féniz | 5 | 1:05.55 | 6 q |
| Camila Aracely Chuquihuaccha Sayritupac | San Francisco College | 6 | 1:11.71 | 19 |
| Ana Lucía Seclen Samanez | Colegio de la Inmaculada Jesuitas | 8 | 1:17.06 | 24 |
| Antonella María Romero De Cunha | Monteprincipe | 3 | DNS | |
| Karelly Sanchez Tantalean | Team Chile | 7 | DNS | |
