| Serie 1 |
| Ian Piero Irishi Matos Tsukazan | AELU | 7 | 53.42 | 2 q |
| Antonio Ramón Camacho Di Giorgi | Academia de Barranco | 5 | 56.86 | 8 q |
| Piero Guillermo Ricci Verastegui | Asociación Deportiva Team Valiente | 8 | 58.56 | 11 |
| Sebastian Federico Wiese Cremer | Regatas | 6 | 58.81 | 12 |
| Jose Manuel Hancco Mendoza | Multisports | 1 | 1:00.85 | 18 |
| Anthony Gabriel Cota Anci | Escuela Formación Deportiva | 2 | 1:03.30 | 25 |
| Adlerk Alarikc Sanchez Salas | Team Chile | 3 | 1:17.07 | 27 |
| Enzo Bastian Fernando Tarazona Lizano | Olimpia AQP | 4 | DNS | |
| Serie 2 |
| Camilo Carlos Vera Raiser | San Francisco College | 3 | 54.82 | 4 q |
| Yukio Kanashiro Oliveros | Nuestra Señora del Carmen | 7 | 55.80 | 6 q |
| Matias Alejandro Bazan Miranda | Atlético Talentos Santos | 1 | 59.53 | 14 |
| Ronaldinho Roselló Ccasa | Coar Deportivo Ica | 6 | 1:02.22 | 21 |
| Carlos Alberto Freitas Garcia | Deportivo Estudiantil República de Venezuela | 2 | 1:02.84 | 24 |
| Ricardo Fabiano Landeo Castro | Alborada Track and Field | 4 | DNS | |
| Bruno Gustavo Acosta Lam | Lord Byron - Arequipa | 5 | DNS | |
| Serie 3 |
| Diego Acuña Basauri | G Coach SAC | 4 | 58.48 | 10 |
| Aaron Amilcar Lopez Del Águila | Deportivo Estudiantil República de Venezuela | 2 | 59.11 | 13 |
| Lucas Leonardo Salazar Ochoa | Lord Byron - Arequipa | 3 | 1:00.04 | 17 |
| Dayron Guido Huaman Guarniz | Apolo Athletic Club | 7 | 1:04.21 | 26 |
| Alexander José Blanco Tovar | Tipiani Team Ica | 5 | DNS | |
| Julio Fernando Arguedas Cespedes | Asociación Deportiva Team Valiente | 6 | DNS | |
| Serie 4 |
| Gonzalo Cartagena Estremadoyro | Marcon Athletic Club | 4 | 54.51 | 3 q |
| Oscar Alonzo Ramos Vassallo | Deportivo Ojeda's Team | 2 | 55.24 | 5 q |
| Hector Sosaya Sosa | Turicará | 8 | 59.88 | 15 |
| Luis Joaquin Benavente Vera | Atlético Talentos Santos | 3 | 1:01.88 | 20 |
| Franco Esteban Canova Raymundo | Desarrollo Deportivo SAC | 6 | 1:02.42 | 22 |
| Adryan Prieto Cevallos | Turicará | 5 | DNF | |
| Diego David Sauñe Figueroa | Atlético Asociación San Isidro de Chipre | 7 | DNS | |
| Serie 5 |
| Giancarlo Andre Bravo Banda | Widax | 8 | 53.24 | 1 q |
| Antonio Costa Hernandez | Franklin Delano Roosevelt | 7 | 56.38 | 7 q |
| Cesar Chafloque Montalvan | Atletismo Sican | 6 | 57.46 | 9 |
| Juan Leonel Villegas Garcia | Atletismo Chiclayo | 2 | 59.92 | 16 |
| Anthony Rai Mori Celis | Multisports | 3 | 1:01.32 | 19 |
| Fabrizio Jafeth Cajamarca Orihuela | San Francisco de Sales | 5 | 1:02.73 | 23 |
| Antonio García Palacios | Alborada Track and Field | 4 | DNS | |
